BT 222 CELL AND GENE THERAPY

This course teaches state-of-the-art strategies, techniques, and approaches employed in the ever-expanding cell and gene therapy field, biomanufacturing process, and biopharma R&D. After completing this course, students will be familiar with critical analytical techniques like -PCR, qPCR, dd PCR, SDS - PAGE, ELISA, SAGE, NGS, DNA/RNA/Protein therapeutics, drug target identification, various drug and gene delivery methods, viral-based drug delivery (Electroporation, CRISPR, Lentivirus), knowledge of Potency assay, Pre-clinical & Clinical trials, production and purification of therapeutic substance, cGMP quality and compliance strategy, Stem cell culture, Flow cytometry, FACS, Ion-exchange chromatography, HPLC, and AKTA filtration. Emphasis is placed on the -Aseptic techniques, Cell culture expansion, Transfection, Production, and purification of Baculovirus, Adeno Associated Virus (AAV) - mediated transduction of CHO cells, HEK- 293 cells, and insect cells (sf9 cells). Lab: 6 hours per week. 3 Credits Prerequisites: BI 110, BT 101, and BT 201