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Dec 22, 2024
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2017-2018 Catalog [ARCHIVED CATALOG]
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BT 215 GENE EXPRESSION LABORATORY COURSE
This course teaches state-of-the-art strategies and techniques employed in the field of gene regulation and gene expression. Emphasis is placed on the analysis of the regulation of gene expression at the transcriptional and post-translational levels and teaches the novel methodologies used to study these processes such as qRT-PCR, RNAi for specific knock-down experiments, mRNA turnover and stability analysis using Northern-blot analysis, RNA primer extension, serial analysis of gene expression (SAGE), and Western Blot analysis. Emphasis will be placed on teaching students on the advantages and limitations of expressiona analysis techniques taught in the class their use in the development of methadologies that inhibit or induce gene expression. 3 Credits. Lab: 6 hours per week. Prerequisites: CH 110, BI 110, BT 101. 3 Credits Prerequisites: BT101 and BI110
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